Gas Mixer for Live Cell Imaging – Microscope Incubator

HOW TO CREATE DYNAMICAL GAS MIXTURES FOR MICROSCOPE INCUBATORS IN REAL TIME.

To deeply research and understand the biological life-cycle of a living cell in real-time, and to be able to analyze the development, or even to correct it along the way, it is required a dynamic microscope incubator system, also called “Live Cell Imaging Microscopes“.
This tool permits a visualization and data analysis of the living cellular and sub-cellular structures, functions, and organization by time-lapse photography.

Live cell imaging is a fundamental system to be used for a large series of scientific research and studies, for example, the response of the human cell to new drugs.
Microscopy techniques can widely vary depending on the requirements, in any case, to keep alive, or even to extend the length of living-time of the cell cultures, it is crucial to keep under control the atmosphere and the environmental conditions. 

When the scope of the studies requires a certain percentage of gases in the atmosphere, a pre-mixed cylinder connected to the incubator can constantly flow a certain quantity of a gas mixture. But, what if the conditions need some adjustment along the way?
To create dynamical Gas Mixtures in real-time can be used a Gas Mixer, also called Gas Blender. A small box capable to connect and mix reactively a range of gases allowing manual or automated fast microflow settings with stability and precision. So on, playing with the input values of O2, CO2, or even many other gases, it becomes possible to monitor a series of living cell images at designated time intervals and bring forward a time course analysis to discover a variety of cell dynamics with a single setting.

APPLICATIONS OF LIVE CELL RESEARCHES:

TIME-LAPSE IMAGING
Time-lapse microscopy can be used to monitor cells over time. More specifically, live cell imaging is the system used to keep alive the cultured cells while studying and recording the dynamic developments in real-time. Depending on the scope of the research, different tools can be used to achieve the purpose.

SUB-CELLULAR STUDIES
Microscopes can help to study the structure, function, and behavior of sub-cells. These kinds of research allow deep learning of cell behaviors and the understanding of organisms, and they are a fundamental key for biomedical matters such as cancer and other human diseases.

DRUG RESEARCHES
Drug development aims to save lives and needs a huge effort in terms of research and safety. All along the way a drug must satisfy the regulatory requirements. These generally constitute a number of tests designed to determine the effectiveness of the drug or major toxicities prior to first use in humans. Cells imaging studies are an essential first step.

CANCER RESEARCHES
Cancer research studies the behavior of tumor cells in any of their aspects. Often cultured cells are immersed in hypoxia, hypercapnia, or different conditions to monitor their reactions and development. These researches aim to figure out the mechanisms of cancer progression and transform the result into concepts that can be applicable to the treatment and prevention of cancer.

CELL MIGRATION ASSAYS
Cell migration is a fundamental process in the development and maintenance of multicellular organisms. The migration is usually monitored by using microscopes. Time-lapse microscopies are used to generate videos of the migrating cells, and later on, speed them up to study the behavior.

3D LIVE CELL ASSAYS
A 3D cell culture is an environment built-up to permit biologically cultured cells to develop in all three dimensions, simulating the “real-life” behaviors or what they would in vivo. These 3d cultures are usually grown in bioreactors, small capsules in which the cells can grow into spheroids or 3D cell colonies.

Much more…

EASY CONNECTED AND READY TO USE:

A simple connection system (USB, WiFi, Bluetooth, 485Bus) and excellent compatibility and integration (Matlab, Profibus, Modbus, Python) make the installation of this tool an elementary task. A ready-to-use and user-friendly software is supplied too, and deployed in a simplified version on the device itself and operable through a responsive touch screen.

The main advantage to mix gases using a gas mixer device is stable, compelling, and cost-effective control over the chamber conditions and a reliable reaching of the achievement of the purposes.

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